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Related Experiment Videos

Target cells modulate dopamine transporter gene expression during brain development

C Perrone-Capano1, A Tino, U di Porzio

  • 1International Institute of Genetics and Biophysics, CNR, Naples, Italy.

Neuroreport
|May 9, 1994
PubMed
Summary
This summary is machine-generated.

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Dopamine transporter (DAT) gene expression in developing rat neurons begins later than other related genes. Striatal target cells enhance DAT gene transcription, regulating a crucial step in dopamine neurotransmission development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Dopaminergic (DA) neurons are crucial for motor control and reward.
  • The dopamine transporter (DAT) is a key regulator of dopaminergic neurotransmission.
  • Understanding the developmental regulation of DAT is essential for comprehending DA system maturation.

Purpose of the Study:

  • To analyze the developmental expression profile of the dopamine transporter (DAT) gene in rat mesencephalic DA neurons.
  • To compare DAT gene expression with other key neuronal markers during prenatal and postnatal development.
  • To investigate the influence of target cells on DAT gene transcription during development.

Main Methods:

  • Analysis of gene expression using quantitative methods.

Related Experiment Videos

  • Comparison of DAT transcripts with tyrosine hydroxylase, neuronal GABA transporter, and synaptic vesicle monoamine transporter genes.
  • In vitro coculture experiments with mesencephalic DA neurons and striatal target cells.

    • Main Results:

    • DAT transcripts were undetectable until embryonic day 15 (E15).
    • Other analyzed genes (tyrosine hydroxylase, GABA transporter, monoamine transporter) were present by embryonic day 12 (E12).
    • Coculture with striatal cells increased DAT gene transcription in E13 DA neurons.

    Conclusions:

    • Striatal target cells regulate DAT gene expression at the transcriptional level.
    • This regulation is a key step in the developmental process of dopaminergic neurotransmission.
    • Developmental timing of DAT expression is influenced by interactions with target neurons.