Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Decrease in myocardial ryanodine receptors and altered excitation-contraction coupling early in the development of

D E Vatner¹, N Sato, K Kiuchi

¹Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston, Mass.

|September 1, 1994

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Production method of millimeter-wave absorber with 3D-printed mold.

The Review of scientific instruments·2020

Same author

Measurement of <math></math> production with the hadronically decaying boson reconstructed as one or two jets in <i>pp</i> collisions at <math> </math> with ATLAS, and constraints on anomalous gauge couplings.

The European physical journal. C, Particles and fields·2020

Same author

Study of <math></math> and <math></math> production in <math></math> collisions at <math> </math> and search for anomalous quartic gauge couplings with the ATLAS experiment.

The European physical journal. C, Particles and fields·2020

Same author

Identification and rejection of pile-up jets at high pseudorapidity with the ATLAS detector.

The European physical journal. C, Particles and fields·2020

Same author

Measurement of detector-corrected observables sensitive to the anomalous production of events with jets and large missing transverse momentum in <math></math> collisions at <math> </math> TeV using the ATLAS detector.

The European physical journal. C, Particles and fields·2020

Same author

Determination of the strong coupling constant <math></math> from transverse energy-energy correlations in multijet events at <math> </math> using the ATLAS detector.

The European physical journal. C, Particles and fields·2020

Rapid ventricular pacing impairs heart contractility early, affecting calcium release mechanisms. These changes in excitation-contraction coupling are crucial in heart failure development.

Area of Science:

Cardiology
Physiology
Molecular Biology

Background:

Rapid ventricular pacing (RVP) initially reduces myocardial contractile function in conscious dogs.
Prolonged RVP (4-7 weeks) exacerbates contractile dysfunction, leading to overt congestive heart failure signs.

Purpose of the Study:

To investigate the early changes in excitation-contraction coupling during RVP-induced heart failure.
To determine the role of sarcoplasmic reticulum calcium release in contractile abnormalities.

Main Methods:

Assessed mechanical restitution and postextrasystolic potentiation as indices of calcium release.
Measured the response to isoproterenol infusion.
Quantified ryanodine receptor and dihydropyridine binding densities and affinities.

Related Experiment Videos

Main Results:

Mechanical restitution and postextrasystolic potentiation were depressed after 1 day of RVP.
Isoproterenol response was significantly blunted (52%) after 1 day of RVP.
3H-ryanodine receptor binding density decreased significantly after 1 day of RVP and remained low during heart failure.
Ryanodine receptor affinity and dihydropyridine binding were unchanged.
Physiological responses and ryanodine receptor binding recovered within 5 days after cessation of 1-day RVP.

Conclusions:

Early alterations in excitation-contraction coupling, particularly involving the sarcoplasmic reticulum calcium release channel, occur during heart failure development.
These early changes are critical in the pathogenesis of contractile dysfunction in heart failure.