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Separation of hematopoietic progenitor cells from human umbilical cord blood
A Nagler1, M Peacock, M Tantoco
1Department of Bone Marrow Transplantation, Hadassah University, Jerusalem, Israel.
Human umbilical cord blood (HUCB) is a rich source of hematopoietic progenitor cells (HPC). Nevertheless, it has been previously reported that attempts to enrich for HPC resulted in substantial loss of progenitor cells. We have developed a cell processing technique for HUCB using 3% gelatin sedimentation and the AIS MicroCELLectorSBA and CD34. By this technique, we were able to deplete red blood cells (RBC) and obtain a partial T cell depletion, while maintaining 70-90% of the HPC. The results indicate that it may be possible to manipulate cord blood without significant loss of HPC, thus enhancing its utility for transplantation.
Human umbilical cord blood (HUCB) is a rich source of hematopoietic progenitor cells (HPC). Nevertheless, it has been previously reported that attempts to enrich for HPC resulted in substantial loss of progenitor cells. We have developed a cell processing technique for HUCB using 3% gelatin sedimentation and the AIS MicroCELLectorSBA and CD34. By this technique, we were able to deplete red blood cells (RBC) and obtain a partial T cell depletion, while maintaining 70-90% of the HPC. The results indicate that it may be possible to manipulate cord blood without significant loss of HPC, thus enhancing its utility for transplantation.