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Immunomagnetic positive selection and colony culture of CD34+ cells from blood
P Law1, L Ishizawa, C van de Ven
1Baxter Healthcare Corporation, Immunotherapy Division, Santa Ana, CA 92705.
Journal of Hematotherapy
|January 1, 1993
Abstract:
Immunomagnetic separation has been used to enrich CD34-positive cells in umbilical cord blood. Cell purities were increased from 0.59% preseparation to 92.7% postseparation (n = 16) with a mean yield of 75.7%. CFU were enriched 127 fold by immunomagnetic separation. Addition of combinations of recombinant growth factors resulted in cloning efficiencies of greater than 50%.