Related Experiment Videos
Isolation of human CD34+ peripheral blood stem cells using an immunomagnetic positive selection system: prior
Y Nakamura1, M Noma, M Kidokoro
1Blood Transfusion Center, Tokai University Hospital, Kanagawa, Japan.
Journal of Hematotherapy
|January 1, 1994
Abstract:
An immunomagnetic separation system has been used to collect CD34+ cells in mobilized blood after treatment with a nylon-wool column. Cell purities were increased from 2.6% preseparation to 94.6% postseparation, with a mean yield 45.2% (n = 4). Forty percent of CD34+ cells separated by the immunomagnetic procedure formed colonies in the presence of hematopoietic growth factors in a limiting dilution assay. Eleven percent of these clones proliferated to over 10(5) cells and contained megakaryocytes.