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[Modification of a panoptic method of staining isolated cells]
E Paulíková1, A Kociková, M Sabol
1Katedra fyziológie zivocichov a cloveka, PrF UPJS v Kosiciach.
Abstract:
A simple and rapid method of isolated cell staining was achieved by modification of the Pappenheim's method. It is based on the following steps: (1) dilution of commonly used stock solutions May-Grünwald (1:1), Giemsa-Romanowsky (1.25% v/v), (2) reverse sequence of the used staining solutions in comparison with the original method, (3) shorter time of procedure (5 min/slide), (4) utilization of distilled water of pH 5.1-5.6 for dilution of solutions and rinsing of slides, (5) it is not necessary to add serum to cell suspensions. The presented histological method is suitable for cytomorphological examination of cells. It can be applied in routine hematological laboratories not equipped with cytospins. (Fig. 5, Ref. 6.).
Insights
This study presents a simplified Pappenheim
Area of Science:
- Hematology
- Histology
- Cell Biology
Context:
- Traditional Pappenheim staining methods can be time-consuming and require specific equipment.
- Cytomorphological examination is crucial for diagnosing various hematological conditions.
- Routine laboratories often lack advanced equipment like cytospins.
Purpose:
- To develop a rapid and simple cell staining method.
- To adapt the Pappenheim staining technique for routine hematological laboratories.
- To enable cytomorphological examination without specialized equipment.
Summary:
- A modified Pappenheim staining method is described, involving diluted May-Grünwald and Giemsa-Romanowsky solutions.
- The protocol reverses the staining sequence, reduces procedure time to 5 minutes per slide, and uses buffered distilled water.
- Serum is not required for cell suspensions, simplifying the process.
Impact:
- Provides a faster and more accessible method for cell staining.
- Facilitates cytomorphological analysis in hematological diagnostics.
- Enhances the capabilities of routine laboratories lacking advanced instrumentation.