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Immunohistochemical margin control applied to Mohs micrographic surgical excision of dermatofibrosarcoma protuberans
F J Jimenez1, J M Grichnik, M D Buchanan
1Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Background:
Surgical treatment of dermatofibrosarcoma protuberans has a high rate of recurrence presumably secondary to persistent residual tumor. Recently an antigenic marker, CD34, has demonstrated specificity for this tumor.
Objective:
To improve the microscopic detection of dermatofibrosarcoma protuberans tumor elements in Mohs micrographic surgical sections by incorporating immunohistologic staining.
Methods:
Standard Mohs micrographic surgical technique was used, coupled with standard immunohistochemical procedures using an antibody to the CD34 antigen.
Results:
Immunohistochemical staining with anti-CD34 of Mohs micrographic sections clearly delineated the extent of the tumor elements.
Conclusions:
We anticipate that the application of this immunohistochemical-modified Mohs surgical technique will further enhance the detection of insidious portions of tumor thereby enhancing removal and reducing recurrence.