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Related Experiment Videos

CD7 is associated with CD3 and CD45 on human T cells

A I Lazarovits1, N Osman, C E Le Feuvre

  • 1Cell Surface Biochemistry Laboratory, Imperial Cancer Research Fund, London, UK.

Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1994
PubMed
Summary

The CD7 molecule, found on human T cells, associates with a tyrosine kinase, CD45, and CD3/TCR. This complex provides a physical basis for CD7

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The CD7 antigen is a 40-kDa glycopolypeptide present on a major subset of human T cells.
  • CD7 antigen mediates an accessory pathway of T cell activation, with cross-linked CD7 monoclonal antibodies (mAbs) being mitogenic.
  • Signals delivered via CD7 antigen stimulate integrin-mediated adhesion, highlighting its role in T cell activation.

Purpose of the Study:

  • To investigate the molecular associations of the CD7 molecule.
  • To elucidate the physical basis for the accessory role of CD7 in T cell activation.

Main Methods:

  • Immunoprecipitation of CD7, CD3, and CD45 from human T cell lysates.
  • In vitro phosphorylation assays using immunoprecipitates.
  • Surface iodination of T cells followed by immunoprecipitation and Western blotting.

Related Experiment Videos

  • Fluorescence resonance energy transfer (FRET) experiments.

    • Main Results:

    • The CD7 molecule is associated with a tyrosine kinase, with CD45 identified as a major substrate.
    • CD7 immunoprecipitates contained CD45 and CD3, confirmed by Western blotting and FRET.
    • Evidence suggests CD7 exists as a homodimer and forms an oligomeric complex with CD3/TCR, CD45, and a tyrosine kinase.

    Conclusions:

    • CD7 forms a physical complex with CD3/TCR, the protein tyrosine phosphatase CD45, and a tyrosine kinase.
    • This molecular complex provides a mechanistic explanation for the accessory function of CD7 in T cell activation.
    • The findings support a model where CD7 plays a crucial role in signal transduction pathways governing T cell responses.