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Updated: Aug 11, 2026
Generation of Human CD40-activated B cells
Published on: October 16, 2009
Human monoclonal anti-D secreting heterohybridomas from peripheral B lymphocytes expanded in the CD40 system
J M Thompson1, J Lowe, D F McDonald
1Research and Development Laboratory, West Midlands Blood Transfusion Service, Edgbaston, Birmingham, UK.
Peripheral human B lymphocytes isolated from four immunised anti-D donors have been cultured in the CD40 system (Banchereau et al., 1991), prior to fusion to murine X63Ag8.653 plasmacytoma cells. High fusion efficiency was noted in all cases and immunoglobulin (IgG and IgM) was detected in nearly all heterohybrid containing wells. Only fusions using boosted donor lymphocytes generated specific anti-D secreting hybrids. A short period of culture of committed B cell precursors in the CD40 system before fusion appears to be both adequate and efficient in permitting generation of specific antibody-secreting hybrids.
Peripheral human B lymphocytes isolated from four immunised anti-D donors have been cultured in the CD40 system (Banchereau et al., 1991), prior to fusion to murine X63Ag8.653 plasmacytoma cells. High fusion efficiency was noted in all cases and immunoglobulin (IgG and IgM) was detected in nearly all heterohybrid containing wells. Only fusions using boosted donor lymphocytes generated specific anti-D secreting hybrids. A short period of culture of committed B cell precursors in the CD40 system before fusion appears to be both adequate and efficient in permitting generation of specific antibody-secreting hybrids.
