Alterations in the glomerular charge barrier in human lupus nephritis

Renal tissue taken from ten patients with proteinuria and diagnosed as suffering from human lupus nephritis was examined by a post-embedding charge labelling method using cationic gold (CG). The method allows localization of the anionic charge in the glomerular epithelial cell coat and/or glomerular basement membrane (GBM). Our results demonstrate that in human lupus nephritis there are gaps in the charge barrier associated with immune deposits found in the lamina rara externa of the GBM. These data suggest that the loss of glomerular fixed anionic charges in the GBM may play an important role in the development of proteinuria in patients suffering from lupus nephritis.