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Induction of chromosomal aberrations with benzon nuclease in Chinese hamster ovary (CHO) cells
1University GH Essen, Department of Genetics, Germany.
Mutation Research
|November 1, 1994
Abstract:
Benzon nuclease, an endonuclease originating from Serratia marcescens, was tested for its chromosome breaking activity in Chinese hamster ovary cells. Using a permeabilizing method with hypertonic glycerol, benzon nuclease induced chromosomal aberrations in an S-phase independent manner. The frequencies of polycentric chromosomes were correlated with the dose of the enzyme and the intercellular distribution of aberrations was overdispersed.