Related Experiment Videos
Database on the structure of small ribosomal subunit RNA
Y Van de Peer1, I Van den Broeck, P De Rijk
1Departement Biochemie, Universiteit Antwerpen (UIA), Belgium.
Nucleic Acids Research
|September 1, 1994
Summary
This study presents a database of 2824 small ribosomal subunit RNA sequences, aligned by secondary structure. The data is accessible for researchers, aiding in the study of ribosomal RNA structure and evolution.
Area of Science:
- Molecular Biology
- Bioinformatics
- Structural Biology
Background:
- Small ribosomal subunit RNA (srRNA) is crucial for protein synthesis.
- Understanding srRNA structure is key to deciphering its function.
- A comprehensive, aligned database facilitates comparative analysis.
Purpose of the Study:
- To compile and align a large dataset of srRNA nucleotide sequences.
- To provide researchers with an accessible resource for studying srRNA structure.
- To support investigations into evolutionary relationships and functional mechanisms.
Main Methods:
- Collection of 2824 srRNA nucleotide sequences.
- Alignment of sequences based on a validated secondary structure model.
- Corroboration of the secondary structure model using compensating substitutions.
- Database distribution via anonymous ftp and the EMBL nucleotide sequence library.
Main Results:
- A curated database containing 2824 aligned srRNA sequences is established.
- The alignment is based on a robust secondary structure model.
- Compensating substitutions provide evidence for the accuracy of the structural model.
- The database is readily available to the scientific community.
Conclusions:
- The database serves as a valuable resource for molecular biologists and bioinformaticians.
- Accessible srRNA sequence data promotes research in ribosomal function and evolution.
- The alignment and structural information enhance the utility of the sequence data.