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Lymphocyte function-associated antigen 1 overexpression and T cell autoreactivity
B Richardson1, D Powers, F Hooper
1University of Michigan, Ann Arbor.
Arthritis and Rheumatism
|September 1, 1994
Objective:
To determine if DNA methylation inhibitors make T cells autoreactive by inducing lymphocyte function-associated antigen type 1 (LFA-1) (CD11a/CD18) overexpression.
Methods:
T cell clones were treated with 3 distinct DNA methylation inhibitors or were stably transfected with a CD18 cDNA in a mammalian expression vector, and the effects on LFA-1 expression and activation requirements were examined.
Results:
LFA-1 overexpression, caused by DNA methylation inhibitors or by transfection, correlates with the development of autoreactivity.
Conclusion:
LFA-1 overexpression may contribute to T cell autoreactivity.