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Related Experiment Videos

Susceptibility of C5b-9(m) to postmortem changes

H Thomsen1, H Held

  • 1Institute of Forensic Medicine, Christian-Albrechts University, Kiel, Germany.

International Journal of Legal Medicine
|January 1, 1994
PubMed
Summary

Complement C5b-9(m) can be detected in myocardial infarction for up to 11 days, even with putrefaction. This immunohistochemical marker remains reliable when standard methods fail in forensic cases.

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Area of Science:

  • Forensic Pathology
  • Immunohistochemistry
  • Cardiovascular Pathology

Background:

  • Complement C5b-9(m) is a specific marker for myocardial cell necrosis.
  • Its diagnostic utility in forensic settings is threatened by postmortem changes like putrefaction and autolysis.
  • Timely diagnosis of myocardial infarction is crucial in forensic investigations.

Purpose of the Study:

  • To evaluate the reliability of immunohistochemical demonstration of C5b-9(m) in necrotic myocardium under conditions of experimentally induced putrefaction and autolysis.
  • To determine the duration for which C5b-9(m) can be reliably detected postmortem.
  • To assess the potential for false positive staining.

Main Methods:

  • Experimental induction of putrefaction and autolysis in myocardial tissue.
  • Immunohistochemical staining for C5b-9(m) on affected myocardium.
  • Comparison with standard Hematoxylin-Eosin staining for myocardial infarction.
  • Assessment of staining reliability and specificity.

Main Results:

  • C5b-9(m) was successfully demonstrated immunohistochemically in necrotic myocardium up to 11 days post-induction of putrefaction and autolysis.
  • This detection was possible even when Hematoxylin-Eosin staining for myocardial infarction was no longer reliable.
  • No false positive immunohistochemical staining for C5b-9(m) was observed under the study's experimental conditions.

Conclusions:

  • Immunohistochemical detection of C5b-9(m) is a robust method for diagnosing myocardial infarction in forensic cases, persisting beyond the reliability of conventional histological methods.
  • C5b-9(m) serves as a valuable and specific marker for myocardial necrosis, even in advanced stages of postmortem decomposition.
  • This technique enhances the diagnostic capabilities in forensic pathology when dealing with challenging postmortem samples.

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