Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene family encoding basic pathogenesis-related 1 proteins in barley

A Mouradov1, E Mouradova, K J Scott

  • 1Department of Biochemistry, University of Queensland, Brisbane, Australia.

Plant Molecular Biology
|October 1, 1994
PubMed
Summary

Researchers identified new barley basic PR-1 proteins (prb1-2 and prb1-3) and found their genes are activated in response to powdery mildew infection in Hordeum vulgare.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extended timescale 2D IR probes of proteins: p-cyanoselenophenylalanine.

Physical chemistry chemical physics : PCCP·2017
Same author

Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia.

Leukemia·2015
Same author

Controlled infection with a therapeutic virus defines the activation kinetics of human natural killer cells in vivo.

Clinical and experimental immunology·2014
Same author

Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus.

Plant molecular biology·2013
Same author

cDNA cloning of mRNAs induced in resistant barley during infection by Erysiphe graminis f.sp. Hordei.

Plant molecular biology·2013
Same author

Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog).

Plant cell reports·2013

Area of Science:

  • Plant molecular biology
  • Plant pathology
  • Genetics

Background:

  • Basic PR-1 proteins are involved in plant defense mechanisms.
  • Hordeum vulgare (barley) is a major cereal crop susceptible to various pathogens.
  • Erysiphe graminis f. sp. hordei causes powdery mildew, a significant disease in barley.

Purpose of the Study:

  • To isolate and characterize new barley basic PR-1 proteins.
  • To investigate the gene family size of barley PR-1 proteins.
  • To examine the expression patterns of these genes upon pathogen challenge.

Main Methods:

  • Isolation of genomic (prb1) and cDNA clones (PRb1-2, PRb1-3).
  • Genomic DNA analysis to determine gene family size.
  • Gene expression analysis in barley leaf tissues after inoculation with Erysiphe graminis f. sp. hordei.

Related Experiment Videos

Main Results:

  • Two new barley basic PR-1 proteins, prb1-2 and prb1-3, were identified.
  • The barley genome contains at least six genes encoding PR-1 proteins.
  • Expression of these PR-1 genes was induced in response to Erysiphe graminis f. sp. hordei infection in both resistant and susceptible barley cultivars.

Conclusions:

  • The identified PR-1 proteins are new components of barley's defense system.
  • The PR-1 gene family in barley is larger than previously known.
  • PR-1 gene expression is a common response to powdery mildew in barley, regardless of resistance gene presence.