Growth factor effects on the proliferation of different retinal glial cells in vitro

Vascularized mammalian retinae contain two distinct neuroglial cells types, radially oriented Müller cells and astrocytes, which are located in the nerve fiber layer. These cell types derive from different precursor cells and proliferate during ontogenesis at distinct schedules. The aim of the present study was to disclose whether growth factors, which are known to interfere with the development of neuroglial cells in the central nervous system, like basic and acidic fibroblast growth factor (aFGF and bFGF), epidermal growth factor (EGF) and platelet-derived growth factor, have similar or distinct effects on the proliferative capacity of retinal astrocytes and Müller cells. These questions were tested by applying growth factors to cultured astrocytes and Müller cells from early postnatal rabbit retina. Proliferating cells were identified by double labeling experiments combining cell type specific markers with bromodeoxyuridine immunocytochemistry and [3H]thymidine incorporation experiments, respectively. In addition, we used the anatomical advantage of the rabbit retina. Its peripheral part is astroglial cell-free. Cultures prepared from this part of the retina (P-cultures) contain Müller cells, microglial cells and neurons, while cultures from the 'central part', the medullary rays (MR) region contain, in addition, astrocytes and oligodendrocytes. Our studies show that Müller cell proliferation is stimulated by EGF in a dose dependent manner, while astrocyte proliferation is stimulated by aFGF and bFGF. The proliferation of O4-positive glial precursor cells is stimulated by aFGF, bFGF and platelet-derived growth factor, but not by EGF. Microglial cells, which are a minor population in these cultures, do not respond to either of these factors.