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Staining of human telomeres with primed in situ labeling (PRINS)
A J Therkelsen1, A Nielsen, J Koch
1Institute of Human Genetics, Aarhus University, Denmark.
Cytogenetics and Cell Genetics
|January 1, 1995
Abstract:
As described, the PRINS method is a very rapid and reliable way of staining human telomeres. To obtain the maximum frequency of stained telomeres, the primer (CCCTAA)7 should be used, although the average frequency never quite reaches 100%. The frequency is strongly dependent on the age of the individual, being significantly higher in children and newborns than in adults. A difference between the (CCCTAA)7 primer and the complementary primer is demonstrated and a possible explanation is proposed, namely, that gaps in the C-rich strand cause chain elongation termination after the addition of only one dTTP molecule.