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Related Experiment Videos

Programming gene expression in developing epidermis

C Byrne1, M Tainsky, E Fuchs

  • 1Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.

Development (Cambridge, England)
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

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Mouse epidermal development reveals early keratin gene expression linked to mesenchymal cues and proliferation. AP2 transcription factor drives basal keratin expression, impacting skin development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Keratins are major proteins in adult keratinocytes and serve as markers for mouse epidermal embryogenesis.
  • Understanding keratin gene expression is crucial for deciphering epidermal development.

Purpose of the Study:

  • To track skin-specific keratin mRNA expression during mouse embryogenesis.
  • To investigate the transcriptional regulation of epidermal gene expression.
  • To understand the role of AP2 in basal keratin gene regulation.

Main Methods:

  • Modified whole-mount in situ hybridization to track endogenous keratin mRNA.
  • Beta-galactosidase expression of a human epidermal keratin promoter-driven transgene to monitor transcriptional regulation.
  • Analysis of AP2 and Sp1 cRNA hybridization patterns.

Related Experiment Videos

  • In vitro studies of AP2's effect on basal keratin expression in cultured cells.

    • Main Results:

    • Basal keratin (K5 and K14) genes are first detected regionally at E9.5, correlating with underlying mesenchyme origin, not morphogenesis.
    • Epidermal keratin genes are expressed in the periderm layer.
    • Later K5 and K14 expression patterns correlate with proliferative capacity, not stratification.
    • K1 and K10 mRNAs appear by E13.5, correlating with differentiation.
    • AP2 cRNAs show patterns similar to, but preceding, basal keratin cRNAs; AP2 strongly induces basal keratin expression in cultured cells.

    Conclusions:

    • Early keratin gene expression patterns are influenced by mesenchymal inductive cues.
    • Periderm arises from ectodermal stratification.
    • K5/K14 expression relates to proliferation, while K1/K10 relates to differentiation.
    • AP2 is a key transcriptional regulator of basal keratin genes during epidermal development.