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Related Experiment Videos

CD44 triggering enhances human NK cell cytotoxic functions

R Galandrini1, R De Maria, M Piccoli

  • 1Department of Experimental Medicine, La Sapienza, University of Rome, Italy.

Journal of Immunology (Baltimore, Md. : 1950)
|November 15, 1994
PubMed
Summary
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CD44 enhances natural killer (NK) cell cytotoxicity against tumor cells. This involves increased intracellular calcium, suggesting CD44 coactivates NK cell functions.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD44 is a major hyaluronate receptor crucial for lymphocyte functions like activation and adhesion.
  • Its role in natural killer (NK) cell cytotoxic functions remains to be fully elucidated.

Purpose of the Study:

  • To investigate the impact of CD44 ligation on human NK cell cytotoxic activity.
  • To explore the early biochemical events following CD44 engagement.

Main Methods:

  • Utilized monoclonal antibodies (mAbs) J173 and F10442 to ligate CD44 on human NK cells.
  • Assessed NK cell cytotoxicity against various tumor target cells.
  • Measured intracellular free calcium levels and inositol 1,4,5-trisphosphate generation after CD44 ligation and cross-linking.

Main Results:

Related Experiment Videos

  • CD44 ligation enhanced NK cell cytotoxicity in a dose-dependent manner against sensitive tumor targets.
  • CD44 cross-linking upregulated CD16-mediated lysis.
  • CD44 engagement induced a rapid increase in intracellular free calcium, enhanced by CD16 coengagement.
  • No inositol 1,4,5-trisphosphate generation was observed upon CD44 cross-linking.

Conclusions:

  • CD44 does not deliver a direct lytic signal but coactivates spontaneous and CD16-mediated NK cell cytotoxicity.
  • The observed increase in intracellular free calcium may mediate this costimulatory effect.