An effective method for depleting mature T lymphocytes from bone marrow cells--two-step Percoll centrifugation

In the present paper we have observed the effect of discontinuous gradient two-step Percoll centrifugation on depleting mature T lymphocytes from normal bone marrow. The pre-/post-Percoll percentage of CD34+, and Leu4+ cells in MNC was counted by using APAAP technique. As the two-step Percoll centrifugation is simple, and time saving, and decreases the incidence of contamination of cultured cells as well, This technique may be of value in serum-free culture of hematopoietic cells and immunological studies.