Enhanced specific-locus mutation response of 101/H male mice to single, acute X-irradiation

The specific-locus mutation yield from 101/H mice following single, acute 6 Gy spermatogonial X-irradiation was significantly higher than both a concurrent C3H/HeH x 101/H F1 hybrid control and historical data obtained with mice of equivalent genotype. There is therefore discord with the reduced translocation response to single X-ray doses previously identified in this strain. By contrast, the mutation yield following a 24-h interval 3 + 3 Gy fractionated X-ray dose was not significantly different from that of its concurrent hybrid control, nor from results obtained by others with mice of the equivalent or different genotypes. Here there is no discord with the translocation response obtained with a 1 + 5 Gy 24-h interval fractionated regime. Appraisal of comparable specific-locus and translocation data indicates that the differing results obtained with the two genetic end-points are not without precedence. This, together with the observation that the responses for cytologically visible deletions and specific-locus mutations are similar, suggests that the latter two events predominantly derive from one-hit events, with translocations deriving from two-hit events and that the probabilities of induction of each type of lesion vary according to the organisation of the nucleus in different phases of the cell cycle.