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Updated: Oct 13, 2026

On-Chip Endothelial Inflammatory Phenotyping
Published on: July 21, 2012
Characterization of binding and TNF-alpha-inducing ability of chitosans on monocytes: the involvement of CD14
M Otterlei1, K M Vårum, L Ryan
1Institute of Cancer Research, University of Trondheim, University Medical Centre (MTS), Norway.
Abstract:
Chitosans with different chemical composition were found to induce TNF-alpha production from human monocytes. Their ability to induce TNF-alpha was found to be highly dependent on neutral-solubility and molecular weight. Monoclonal antibodies against CD14 inhibited TNF-alpha production from monocytes stimulated with neutral-soluble chitosans. Binding studies indicated that lipopolysaccharides (LPS) and neutral-soluble chitosans share a binding site on monocytes which involves CD14. TNF-alpha production from monocytes stimulated with chitosans was dependent on serum. LPS-binding protein (LBP) enhanced the chitosan-induced TNF-alpha production only to a minor degree, suggesting that serum proteins other than LBP play an important role in the stimulatory effect.
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