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Basic fibroblast growth factor identified in chronically stimulated cardiomyoplasties
V F Blood1, M G Magno, W F Bailey
1Department of Surgery, Jefferson Medical College, Philadelphia, Pennsylvania 19107.
The Annals of Thoracic Surgery
|November 1, 1994
Summary
Chronic electrical stimulation of latissimus dorsi (LD) cardiomyoplasty increases extramyocardial collateral blood flow, potentially mediated by basic fibroblast growth factor (bFGF). Studies suggest bFGF is involved in collateral formation in stimulated skeletal muscle.
Area of Science:
- Cardiovascular Research
- Skeletal Muscle Physiology
- Biomedical Engineering
Background:
- Myocardial ischemia compromises blood flow, necessitating strategies to enhance collateral circulation.
- Latissimus dorsi (LD) cardiomyoplasty is a surgical procedure used to augment cardiac function.
- Electrical stimulation of cardiomyoplasty has shown promise in improving blood flow.
Purpose of the Study:
- To investigate the role of basic fibroblast growth factor (bFGF) in mediating enhanced collateral blood flow following chronic electrical stimulation of LD cardiomyoplasty.
- To determine if bFGF is present in skeletal muscle after electrical stimulation.
- To assess the effect of exogenous bFGF administration on endogenous bFGF production.
Main Methods:
- Western blot analysis and immunohistochemistry were used to detect bFGF in LD muscle samples.
- Three groups of goats with LD cardiomyoplasty were studied: static, 2-Hz stimulated, and stimulated with exogenous bFGF.
- Analysis focused on bFGF presence and concentration in different experimental conditions.
Main Results:
- Basic fibroblast growth factor (bFGF)-like immunoreactivity was detected in electrically stimulated LD cardiomyoplasty (group 2) by both Western blot and immunohistochemistry.
- bFGF was present in stimulated goat skeletal muscle, with higher concentrations suggested in chronically stimulated cardiomyoplasties.
- Exogenous bFGF administration did not downregulate, and may have upregulated, endogenous bFGF production.
Conclusions:
- Skeletal muscle basic fibroblast growth factor (bFGF) is present in latissimus dorsi (LD) cardiomyoplasty after chronic electrical stimulation.
- bFGF may play a significant role in the formation of extramyocardial collaterals.
- Exogenous bFGF can be safely administered and may enhance endogenous bFGF production, supporting its therapeutic potential.