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Related Experiment Videos

Intercellular adhesion molecule-1 is a cell surface receptor for hyaluronan

P A McCourt1, B Ek, N Forsberg

  • 1Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.

The Journal of Biological Chemistry
|December 2, 1994
PubMed
Summary

Researchers identified the hyaluronan receptor in rat liver as intercellular adhesion molecule-1 (ICAM-1). This finding reveals ICAM-1

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Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • The hyaluronan receptor on rat liver endothelial cells was previously characterized and purified.
  • Understanding the molecular identity of this receptor is crucial for studying cell adhesion.

Purpose of the Study:

  • To isolate and identify the molecular structure of the hyaluronan receptor from whole rat liver.
  • To determine the amino acid sequence of the purified receptor for identification.

Main Methods:

  • Isolation of the hyaluronan receptor from whole rat liver.
  • Amino acid sequence analysis of peptides derived from the affinity-purified receptor.
  • Comparison of obtained peptide sequences with known protein databases.

Main Results:

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  • Sufficient quantities of the receptor were isolated for amino acid sequence analysis.
  • Four distinct peptides from the purified receptor showed identity with rat intercellular adhesion molecule-1 (ICAM-1).
  • ICAM-1, a glycoprotein, is typically low in endothelial cells but upregulated in inflamed and malignant tissues.

Conclusions:

  • The hyaluronan receptor in rat liver is identified as intercellular adhesion molecule-1 (ICAM-1).
  • ICAM-1 mediates cell-cell adhesion, acting as a ligand for LFA-1 and Mac-1.
  • The binding affinity of ICAM-1 for hyaluronan has significant implications for cell adhesion in health and disease (inflammation, atherosclerosis, cancer).