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Intercellular adhesion molecule-1 is a cell surface receptor for hyaluronan
P A McCourt1, B Ek, N Forsberg
1Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
The Journal of Biological Chemistry
|December 2, 1994
Summary
Researchers identified the hyaluronan receptor in rat liver as intercellular adhesion molecule-1 (ICAM-1). This finding reveals ICAM-1
Area of Science:
- Cell Biology
- Immunology
- Biochemistry
Background:
- The hyaluronan receptor on rat liver endothelial cells was previously characterized and purified.
- Understanding the molecular identity of this receptor is crucial for studying cell adhesion.
Purpose of the Study:
- To isolate and identify the molecular structure of the hyaluronan receptor from whole rat liver.
- To determine the amino acid sequence of the purified receptor for identification.
Main Methods:
- Isolation of the hyaluronan receptor from whole rat liver.
- Amino acid sequence analysis of peptides derived from the affinity-purified receptor.
- Comparison of obtained peptide sequences with known protein databases.
Main Results:
- Sufficient quantities of the receptor were isolated for amino acid sequence analysis.
- Four distinct peptides from the purified receptor showed identity with rat intercellular adhesion molecule-1 (ICAM-1).
- ICAM-1, a glycoprotein, is typically low in endothelial cells but upregulated in inflamed and malignant tissues.
Conclusions:
- The hyaluronan receptor in rat liver is identified as intercellular adhesion molecule-1 (ICAM-1).
- ICAM-1 mediates cell-cell adhesion, acting as a ligand for LFA-1 and Mac-1.
- The binding affinity of ICAM-1 for hyaluronan has significant implications for cell adhesion in health and disease (inflammation, atherosclerosis, cancer).