Substance P immunoreactive projections to the ventral respiratory group in the rat

Retrograde tracing (rhodamine beads) combined with immunohistochemistry was used to determine the origin of neurons containing substance P that project to the rostral ventral respiratory group in the rat. Double-labeled neurons (rhodamine beads plus substance P immunoreactivity) were found in the midline caudal raphe nuclei (raphe obscurus, raphe pallidus, and raphe magnus) and in the ventrolateral medulla in the parapyramidal region. The findings of this study suggest that substance P-containing neurons in the caudal raphe nuclei and parapyramidal region project to inspiratory neurons in the rostral ventral respiratory group and may therefore influence their activity.