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Neutrophil activation after percutaneous transluminal coronary angioplasty
1Third Department of Internal Medicine, Kurume University School of Medicine, Japan.
Insights
Percutaneous transluminal coronary angioplasty (PTCA) activates neutrophils, indicated by increased CD11b expression and elastase. This suggests neutrophils may play a role in the ischemic injury following PTCA procedures.
Area of Science:
- Cardiology
- Immunology
- Biochemistry
Background:
- Coronary artery disease (CAD) affects millions globally.
- Percutaneous transluminal coronary angioplasty (PTCA) is a common intervention for CAD.
- The role of neutrophils in PTCA-induced injury is not fully understood.
Purpose of the Study:
- To investigate whether PTCA induces neutrophil activation in patients with coronary artery disease.
- To assess specific markers of neutrophil activation following PTCA.
Main Methods:
- Blood samples were collected from the coronary sinus of patients undergoing PTCA and coronary arteriography (CAG).
- Flow cytometry was used to measure CD11b expression and hydrogen peroxide generation in neutrophils.
- Neutrophil elastase levels were measured using an immunoenzymatic method.
Main Results:
- PTCA significantly increased CD11b surface expression on neutrophils (approximately twofold).
- PTCA led to a significant decrease in stimulated neutrophil hydrogen peroxide generation, suggesting prior in vivo activation.
- Neutrophil elastase levels significantly increased after PTCA compared to CAG.
Conclusions:
- PTCA induces significant neutrophil activation in patients with coronary artery disease.
- Activated neutrophils may contribute to the ischemic injury associated with PTCA.
- Further research is warranted to explore therapeutic strategies targeting neutrophil activation post-PTCA.
Abstract:
We investigated whether percutaneous transluminal coronary angioplasty (PTCA) would induce neutrophil activation in patients with coronary artery disease. Blood samples were taken from the coronary sinus in 14 patients who underwent PTCA and in 9 control subjects who underwent coronary arteriography (CAG). Flow cytometry was used to measure membrane surface expression of beta 2 integrin (CD11b) and the generation of hydrogen peroxide in neutrophils after ex vivo phorbol myristate acetate stimulation by 2,'7'-dichlorofluorescein. Neutrophil elastase was measured by an immunoenzymatic method. Surface expression of CD11b increased significantly, approximately twofold, after PTCA but not after CAG. Mean fluorescence intensity of 2',7'-dichlorofluorescein in stimulated neutrophils decreased significantly after PTCA, suggesting a previous in vivo activation, but not after CAG. Neutrophil elastase increased significantly after PTCA but not after CAG. These data indicate that PTCA induces neutrophil activation and suggest that neutrophils may contribute to the ischemic injury.