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Analysis of bone marrow stem cell

L W Terstappen1, S Huang

  • 1Becton Dickinson Immunocytometry Systems, San Jose, California 95131.

Blood Cells
|January 1, 1994
PubMed
Summary
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Hematopoietic stem cells (HSCs) were identified in CD34+ cells lacking CD38. These cells, particularly CD34+ CD38- HLA-DR+, show potential for myeloid and lymphoid differentiation in vitro.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Developmental Biology

Background:

  • Hematopoietic stem cells (HSCs) are crucial for lifelong blood cell production.
  • Defining HSC populations is essential for understanding hematopoiesis and developing cell therapies.
  • Fetal bone marrow is a rich source of HSCs for research.

Purpose of the Study:

  • To define and characterize hematopoietic stem cells (HSCs) from fetal bone marrow.
  • To investigate the differentiation potential of specific CD34+ cell subpopulations.
  • To explore in vitro culture conditions supporting HSC growth and differentiation.

Main Methods:

  • Single-cell sorting of four CD34+ fetal bone marrow populations: CD38-HLA-DR-, CD38-HLA-DR+, CD38+ HLA-DR-, and CD38+ HLA-DR+.
  • Culture of sorted cells in media supplemented with various cytokines (IL-3, IL-6, SCF, etc.) and growth factors (bFGF, IGF-1).

Related Experiment Videos

  • Assessment of plating efficiency and lymphoid/myeloid growth potential of different cell populations.

    • Main Results:

    • HSCs were primarily found in CD38-negative populations.
    • The CD34+ CD38- HLA-DR+ population exhibited the highest plating efficiency (48%).
    • A small fraction of CD34+ CD38- HLA-DR+ cells demonstrated both lymphoid and myeloid potential; CD34+ CD38-HLA-DR- cells showed limited growth (1-5%) with bFGF and IGF-1.

    Conclusions:

    • Specific CD34+ cell subsets, particularly those lacking CD38, contain hematopoietic stem cells.
    • Culture conditions influence the growth and differentiation potential of HSCs.
    • These HSCs can differentiate in vitro to form structures mimicking bone formation.