[Kinetics of reactions in multienzyme systems. I. Steady state processes in bienzyme systems. Lineal multienzyme...

Kinetic aspects of reactions in multienzyme systems are discussed. Detailed formal-kinetic discription of bienzyme system is made for the steady-state conditions. Two important cases--constant and variable concentration of initial substrate are discussed. The criteria for realization of steady-state condition of system are formulated. Equations which describe the steady rates and steady state concentrations of intermediates as functions of initial concentrations and kinetic parameters of individual enzymes were developed. The time range of steady-state conditions was estimated and methods for the determination of kinetic parameters of enzymes in steady-state condition were developed. The regulation aspects of bienzyme system are considered. An analysis of reactions kinetics in lineal multienzyme systems was made and criteria of steady-state condition were formulated. Methods of determination of kinetic parameters Vmax and Kmax for each individual enzyme of multienzyme system were developed. The principle of limiting step was formulated quantitatively, criteria and methods of the finding of limiting step were developed.