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Updated: Aug 17, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
Expression and characterisation of a very-late antigen-4 (alpha 4 beta 1) integrin-binding fragment of vascular
T J Dudgeon1, M J Bottomley, P C Driscoll
1British Biotech, Cowley, Oxford, England.
Abstract:
We have used an Escherichia coli expression system to produce forms of vascular cell-adhesion molecule-1 (VCAM-1) containing the first two and three supposed immunoglobulin-like domains. A form consisting of the first two domains of VCAM-1 is shown to promote very-late antigen-4-dependent spreading of a melanoma cell line comparable to that found for the equivalent region in the full seven-domain form. Preliminary structural analysis by CD and NMR is consistent with an immunoglobulin fold which is predicted from sequence comparison studies.
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