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[Analysis of lipopolysaccharide (LPS) from impermeability-type drug resistant Pseudomonas aeruginosa using new,
K O'Hara1, H Fukuda, H Nakahara
1Department of Microbiology, School of Pharmaceutical Sciences, Tokyo College of Pharmacy.
Abstract:
The specific ladder pattern on polyacrylamide gel electropholesis of lipopolysaccharide (LPS) extracted from Pseudomonas aeruginosa was clearly shown by using the complex methods of the PAS staining, re-periodate oxidation and then Ag-staining method. Accordingly, it was concluded that the new method was greatly useful for a detail analysis of LPS changes in Gram-negative bacteria. And it was shown by this method that no changes in LPS occurred between the impermeability-type drug resistant P. aeruginosa mediated by R plasmid and a drug susceptible strain. The absence of changes indicated that the LPS of P. aeruginosa K-Ps102 had not role in the mechanism of the high drug resistance.