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Published on: May 22, 2014
Interleukin-3 administration enhances human monocyte function in vivo
A Khwaja1, I E Addison, B Johnson
1Department of Haematology, University College London Medical School.
British Journal of Haematology
|November 1, 1994
Summary
Interleukin-3 (IL-3) infusion temporarily reduced monocyte and eosinophil counts but enhanced monocyte phagocytosis, killing of Candida, and migration. This suggests IL-3 could boost immune defenses in immunocompromised patients.
Area of Science:
- Immunology
- Hematology
Background:
- Interleukin-3 (IL-3) is known to affect leucocyte function in vitro.
- Its in vivo effects on haematological variables and monocyte function require further investigation.
Purpose of the Study:
- To investigate the effects of a single IL-3 infusion on haematological parameters and monocyte function in healthy individuals.
Main Methods:
- A single IL-3 infusion was administered to five healthy volunteers.
- Haematological variables, monocyte adhesion molecule expression (CD11b, L-selectin), respiratory burst activity, phagocytosis, and candidacidal activity were measured.
- Neutrophil and monocyte migration in a 'skin window' model was assessed.
Main Results:
- A rapid, transient decrease in circulating monocytes and eosinophils was observed post-IL-3 infusion.
- Monocyte phagocytosis of Candida increased significantly (P < 0.05) and candidacidal activity improved significantly (P < 0.01).
- Monocyte migration was moderately enhanced (peak increase of 260%), while neutrophil migration remained unaffected.
Conclusions:
- IL-3 significantly enhances monocyte function in vivo, specifically phagocytosis and candidacidal activity.
- IL-3 may be a potential therapeutic agent for augmenting host defense mechanisms in immunocompromised individuals.
