Localization of nitric oxide synthase immunoreactivity in mast cells of human nasal mucosa
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S Bacci1, R Arbi-Riccardi, B Mayer
1Department of Human Anatomy and Histology, Section E. Allara, University of Florence, Italy.
Histochemistry
|August 1, 1994
Abstract:
Nitric oxide (NO)-synthase immunoreactivity has been detected for the first time in mast cells of human normal nasal mucosa, with an antibody specific for neuronal NO-synthase. Intense immunoreactivity was revealed in secretion granules of mast cells but was found in mast cell granules free in the extracellular matrix only in some instances; no reactivity was found in the cytoplasm of this or other cell types. These findings suggest that human nasal mast cells contain a particulate isoform of NO-synthase, which shares epitopes with neuronal NO-synthase and is rapidly removed from granules upon exocytosis.