Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bone morphogenetic proteins induce differentiation in astrocyte lineage cells

J S D'Alessandro1, J Yetz-Aldape, E A Wang

  • 1Genetics Institute Inc., Cambridge, MA 02140.

Growth Factors (Chur, Switzerland)
|January 1, 1994
PubMed
Summary

Bone Morphogenetic Proteins (BMPs) promote the differentiation of astrocytes, a key glial cell type. These BMPs increase Glial Fibrillary Acidic Protein (GFAP) and cell adhesion molecule expression in developing neural cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Community investment interventions as a means for decarceration: A scoping review.

Lancet regional health. Americas·2023
Same author

Immune profiling of lupus miliaris disseminatus faciei and successful management with anti-tumour necrosis factor therapy.

Clinical and experimental dermatology·2021
Same author

Bilayer free-standing beam splitter for Fourier transform infrared spectrometry.

Applied optics·2010
Same author

Asymptomatic large main pulmonary artery thromboembolism with a low-probability ventilation-perfusion lung scan.

Clinical nuclear medicine·2001
Same author

Recombinant human interleukin-11 synergizes with steel factor and interleukin-3 to promote directly the early stages of murine megakaryocyte development in vitro.

Blood·2000
Same author

Indium-111 pentetreotide scintigraphy of mesenchymal tumor with oncogenic osteomalacia.

Clinical nuclear medicine·1999

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Serum-free mouse embryo (SFME) cells are a model for studying astrocyte differentiation.
  • Glial Fibrillary Acidic Protein (GFAP) is a specific marker for astrocytes.
  • Transforming Growth Factor Beta (TGF-beta) and calf serum induce GFAP expression in SFME cells.

Purpose of the Study:

  • To investigate the effects of Bone Morphogenetic Proteins (BMPs) on astrocyte differentiation.
  • To determine if BMPs can induce GFAP expression in SFME cells.
  • To analyze the expression of cell adhesion molecules in BMP-treated astrocytes.

Main Methods:

  • SFME cells were treated with various BMP heterodimers (BMP-2/6, 2/7) and homodimers (BMP-2, 4, 5, 6, 7).
  • GFAP expression was measured to assess astrocyte differentiation.

Related Experiment Videos

  • Antibody markers were used to characterize astrocyte types (type 1 and type 2).
  • Levels of cell adhesion molecules CD44 and neural cell adhesion molecule (N-CAM) were quantified.

    • Main Results:

    • BMP treatment induced higher levels of GFAP compared to TGF-beta 1 or activin.
    • BMP-induced cells exhibited characteristics of classical astrocytes (type 1 and type 2).
    • Increased expression of CD44 and N-CAM was observed in BMP-treated astrocytes.

    Conclusions:

    • BMPs act as potent differentiation factors for astrocyte lineage cells.
    • BMPs regulate the expression of cell adhesion molecules in astrocytes.
    • BMPs play a significant role in the development of the glial system in the nervous system.