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Related Experiment Videos

L-selectin expression increases on peripheral blood polymorphonuclear leukocytes during active marrow release

S Van Eeden1, R Miyagashima, L Haley

  • 1Pulmonary Research Laboratory, University of British Columbia, St. Paul's Hospital, Vancouver, Canada.

American Journal of Respiratory and Critical Care Medicine
|February 1, 1995
PubMed
Summary

Bone marrow release of polymorphonuclear leukocytes (PMN) during surgery increases L-selectin expression on circulating PMN. Hypothermia during cardiopulmonary bypass prevents this L-selectin upregulation, potentially impacting sepsis pathogenesis.

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Area of Science:

  • Immunology
  • Cell Biology
  • Surgical Science

Background:

  • L-selectin, a cell adhesion molecule, is highly expressed on bone marrow polymorphonuclear leukocytes (PMN).
  • Increased L-selectin on circulating PMN may contribute to multiorgan failure following sepsis and septic shock.
  • The release of PMN from bone marrow into circulation is a key factor to investigate.

Purpose of the Study:

  • To test the hypothesis that PMN release from bone marrow increases L-selectin expression on circulating PMN.
  • To investigate the role of L-selectin in PMN trafficking during cardiopulmonary bypass (CPB).
  • To determine the effect of normothermic versus hypothermic CPB on L-selectin expression.

Main Methods:

  • Peripheral blood and bone marrow samples were collected during CPB procedures.

Related Experiment Videos

  • L-selectin expression was quantified using immunocytochemistry and flow cytometry.
  • Comparison of L-selectin levels on PMN in normothermic and hypothermic conditions.

    • Main Results:

    • Baseline L-selectin expression was higher on bone marrow PMN than peripheral blood PMN.
    • Normothermic CPB increased circulating band cells and elevated L-selectin expression and MFI on PMN.
    • L-selectin expression was higher on band cells than segmented PMN; hypothermia inhibited these changes.

    Conclusions:

    • Bone marrow PMN release during normothermic CPB significantly upregulates L-selectin on circulating PMN.
    • Hypothermia during CPB mitigates the release of PMN and subsequent L-selectin upregulation.
    • Findings suggest a mechanism linking CPB conditions to PMN activation and potential inflammatory responses.