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Related Experiment Videos

Ethanol increases hepatocyte water volume

R Wondergem1, J Davis

  • 1Department of Physiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0576.

Alcoholism, Clinical and Experimental Research
|October 1, 1994
PubMed
Summary
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Ethanol exposure causes mouse hepatocytes to swell, but unlike osmotic stress, it does not alter their transmembrane potential (Vm). This suggests distinct mechanisms for ethanol-induced cell volume changes.

Area of Science:

  • Hepatocyte physiology
  • Cell volume regulation
  • Ethanol toxicity

Background:

  • Osmotic stress induces adaptive changes in hepatocyte transmembrane potential (Vm), influencing ion redistribution and cell volume regulation.
  • Understanding ethanol's effects on hepatocyte volume and Vm is crucial for comprehending liver function and injury.

Purpose of the Study:

  • To investigate whether in vitro ethanol administration increases hepatocyte water volume in mouse liver slices.
  • To determine if ethanol-induced hepatocyte swelling triggers adaptive changes in transmembrane potential (Vm).

Main Methods:

  • Mouse liver slices were used, with cells loaded with tetramethylammonium ion (TMA).
  • Hepatocyte water volume changes were calculated from TMA intracellular activity measurements using ion-sensitive microelectrodes.

Related Experiment Videos

  • Transmembrane potential (Vm) was monitored in control, ethanol-treated, and osmotically stressed hepatocytes.

    • Main Results:

    • Ethanol (70 mM) caused immediate hepatocyte swelling, peaking at 17% within 7-8 minutes.
    • 4-methylpyrazole pretreatment did not alter ethanol's effect on cell volume, only delaying its onset and peak.
    • Ethanol did not induce significant changes in hepatocyte Vm during swelling, unlike hyposmotic stress which caused hyperpolarization.

    Conclusions:

    • Ethanol-induced hepatocyte swelling occurs independently of changes in transmembrane potential (Vm).
    • The mechanism of ethanol-induced cell swelling differs from that of hyposmotic stress-induced swelling.
    • Ethanol's effects on Vm are only observed at high concentrations (≥257 mM), partially inhibiting osmotic stress-induced hyperpolarization.