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Human cutaneous mast cells express basic fibroblast growth factor

J A Reed1, A P Albino, N S McNutt

  • 1Department of Pathology, New York Hospital, Cornell Medical Center, New York.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|February 1, 1995
PubMed
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Basic fibroblast growth factor (bFGF) is found in mast cells (MCs), which also produce heparin. This suggests MCs release active bFGF during wound healing and angiogenesis when heparin is released.

Area of Science:

  • Cell Biology
  • Wound Healing Research
  • Biochemistry

Background:

  • Tissue injury triggers inflammation and repair via chemical mediators.
  • Basic fibroblast growth factor (bFGF) is a key mediator involved in chemotaxis, proliferation, and angiogenesis.
  • The secretion mechanism and in vivo role of bFGF have been unclear, despite its known heparin-binding properties.

Purpose of the Study:

  • To investigate the presence and localization of bFGF within mast cells (MCs).
  • To explore the potential role of MCs in regulating bFGF activity during tissue repair and angiogenesis.

Main Methods:

  • Nucleic acid in situ hybridization to detect bFGF mRNA in MCs.
  • Immunohistochemistry to confirm bFGF protein expression in human skin tissues.

Related Experiment Videos

  • Heparinase treatment to assess the accessibility of bFGF epitopes.

    • Main Results:

    • MCs in all examined tissues expressed bFGF mRNA and protein.
    • bFGF protein detection was enhanced after heparinase treatment, indicating heparin masking.
    • This suggests bFGF is stored intracellularly bound to heparin within MCs.

    Conclusions:

    • Mast cells are involved in tissue repair and angiogenesis through bFGF.
    • Intracellular heparin in MCs likely binds and regulates bFGF.
    • Degranulation of MCs may release biologically active bFGF in vivo, facilitating healing and new blood vessel formation.