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Related Experiment Videos

CD11/CD18 panel report for swine CD workshop

Y B Kim1, J Zhang, W C Davis

  • 1Department of Microbiology and Immunology, University of Health Sciences, Chicago Medical School, IL 60064.

Veterinary Immunology and Immunopathology
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

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Five monoclonal antibodies (mAbs) targeting porcine CD18 were identified. Three mAbs, PNK-I, H20A, and MHM23, bind to the same CD18 epitope, aiding in immune cell research.

Area of Science:

  • Immunology
  • Veterinary Science
  • Cell Biology

Background:

  • Monoclonal antibodies (mAbs) are crucial tools for identifying cell surface markers.
  • Porcine immune cell research requires specific reagents for characterizing leukocyte populations.

Purpose of the Study:

  • To characterize five monoclonal antibodies (mAbs) against porcine CD11/18.
  • To determine the epitope binding sites of these mAbs on porcine peripheral blood mononuclear cells (PBMC) and polymorphonuclear cells (PMN).

Main Methods:

  • Flow cytometry was used to assess the reactivity of five mAbs (PNK-I, H20A, MUC76A, MUC93A, MHM23) with porcine PBMC and PMN.
  • Epitope mapping was performed using competitive binding assays with PNK-I as the CD18 epitope-defining antibody.

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Main Results:

  • All five mAbs reacted with a high percentage (80-96%) of porcine PBMC and PMN.
  • H20A and MHM23 mAbs bind to the same epitope as PNK-I, defining them as CD18a mAbs.
  • MUC76A and MUC93A mAbs bind to distinct epitopes compared to PNK-I.

Conclusions:

  • Three mAbs (PNK-I, H20A, MHM23) were successfully designated as CD18a mAbs due to shared epitope binding.
  • These CD18a mAbs are valuable reagents for studying porcine immune cell populations.
  • Distinct mAbs (MUC76A, MUC93A) offer potential for identifying different CD18 epitopes.