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Mononuclear phagocytic cells in human milk: HLA-DR and Fc gamma R ligand expression
R A Rivas1, A A el-Mohandes, I M Katona
1Newborn Service, George Washington University Hospital, Washington, DC 20037.
Biology of the Neonate
|January 1, 1994
Summary
Human milk macrophages (BMM) share some traits with peripheral blood monocytes (PBM) but show lower expression of Fc gamma receptors, impacting infant immunity. Understanding these cellular immune components is key to infant protection against infection.
Area of Science:
- Immunology
- Cell Biology
Background:
- Human milk's role in infant protection against infection is crucial.
- Cellular immune components in human milk require detailed investigation.
Purpose of the Study:
- To investigate phenotypic characteristics of breast milk macrophages (BMM).
- To compare BMM characteristics with adult peripheral blood monocytes (PBM).
Main Methods:
- Dual parameter flow microfluorometry was used for analysis.
- Expression of monocyte/macrophage markers (Leu-M3, Leu-M5), HLA-DR, and Fc gamma receptors (Fc gamma RI, RII, RIII) were quantified.
Main Results:
- BMM expressed Leu-M3 and Leu-M5, with high density of Leu-M5.
- HLA-DR expression density was higher on BMM than PBM and further enhanced by interferon-gamma.
- Expression of Fc gamma receptors (Fc gamma RI, RII, RIII) was lower on BMM compared to PBM.
Conclusions:
- BMM exhibit distinct phenotypic characteristics compared to PBM.
- Differences in Fc gamma receptor expression on BMM may influence their function in protecting nursing infants.