Human circulating specific antibody-forming cells after systemic and mucosal immunizations: differential homing
M Quiding-Järbrink1, M Lakew, I Nordström
1Department of Medical, Microbiology and Immunology, University of Göteborg, Sweden.
European Journal of Immunology
|February 1, 1995
Summary
Human volunteers
Area of Science:
- Immunology
- Cell Biology
Background:
- Antibody-secreting cells (ASC) are crucial for adaptive immunity.
- Understanding ASC differentiation and homing is key to effective immunization strategies.
Purpose of the Study:
- To characterize circulating ASC induced by mucosal and systemic immunizations in humans.
- To investigate the differentiation stage and homing receptor expression of these ASC.
Main Methods:
- Flow cytometry analysis of peripheral blood ASC from human volunteers post-immunization.
- Characterization of cell surface markers including CD19, HLA-DR, CD38, CD28, CD22, CD37, CD25, CD71, CD44, alpha 4-integrins, and L-selectin.
Main Results:
- The majority of circulating ASC are terminally differentiated B cells, not fully mature plasmocytes.
- Mucosally-induced ASC showed more homogenous differentiation compared to systemically-induced ASC.
- Systemically-induced ASC predominantly expressed L-selectin, while mucosally-induced ASC showed limited L-selectin expression, suggesting distinct homing mechanisms.
Conclusions:
- Circulating ASC exhibit distinct differentiation and homing profiles based on immunization route (mucosal vs. systemic).
- Mucosal B cell precursors utilize organ-specific recognition mechanisms, differing from systemic B cells.
- Receptor expression specialization may underlie the coordinated mucosal immune response and segregation of mucosal immunity.
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