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Related Experiment Videos

Antigen-binding B cells and polyreactive antibodies

Z J Chen1, J Wheeler, A L Notkins

  • 1Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda 20892.

European Journal of Immunology
|February 1, 1995
PubMed
Summary
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Cells that bind antigens are more likely to produce polyreactive antibodies, which can bind to multiple targets. Antigen binding serves as a key indicator for identifying these polyreactive antibody-producing cells.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • B cells are crucial for adaptive immunity, producing antibodies to combat pathogens.
  • Polyreactive antibodies, capable of binding multiple antigens, play a role in innate immunity and self-tolerance.
  • Understanding the characteristics of polyreactive antibody-producing cells is essential for immunological research.

Purpose of the Study:

  • To investigate whether antigen-binding B cells can produce polyreactive antibodies.
  • To identify markers and characteristics of B cells that generate polyreactive antibodies.
  • To explore the frequency and properties of polyreactive antibody production in different B cell subsets.

Main Methods:

  • Incubation of B cells with labeled self and non-self antigens.

Related Experiment Videos

  • Separation of antigen-binding B cells using flow cytometry.
  • Immortalization of B cells with Epstein-Barr virus for clonal analysis.
  • Immunoflow cytometry to assess antibody binding profiles.

    • Main Results:

    • Antigen-binding B cells showed a significantly higher capacity to produce polyreactive antibodies compared to non-binding cells.
    • The majority of polyreactive antibodies were of the immunoglobulin (Ig)M isotype.
    • Polyreactive antibody-producing cell lines bound diverse antigens (e.g., insulin, IgGFc, beta-galactosidase), unlike monoreactive lines.
    • Both CD5+ and CD5- B cells produced polyreactive antibodies, with a higher frequency in the CD5+ subset.

    Conclusions:

    • Cells capable of binding various antigens can indeed produce polyreactive antibodies.
    • Antigen binding is a reliable marker for identifying B cells that produce polyreactive antibodies.
    • This finding contributes to understanding B cell repertoire and antibody diversity.