Related Experiment Videos
Nitric oxide is released in regenerating liver after partial hepatectomy
S Hortelano1, B Dewez, A M Genaro
1Instituto de Bioquímica (Centro Mixto CSIC-UCM), Facultad de Farmacia Universidad Complutense, Madrid, Spain.
Hepatology (Baltimore, Md.)
|March 1, 1995
Summary
Nitric oxide (NO) plays a role in liver regeneration after partial hepatectomy (PH). Studies show NO biosynthesis increases post-PH, influencing DNA ploidy and macrophage activity, suggesting NO
Area of Science:
- Hepatology and Immunology
- Biochemistry and Molecular Biology
Background:
- Partial hepatectomy (PH) triggers complex cellular responses in the liver.
- Understanding the molecular mediators of liver regeneration is crucial for therapeutic development.
Purpose of the Study:
- To investigate the role of nitric oxide synthase (NOS) and nitric oxide (NO) in liver regeneration following PH.
- To explore the involvement of macrophages in NO-mediated responses post-hepatectomy.
Main Methods:
- Assessed NOS activity and messenger RNA (mRNA) levels in liver remnants post-PH.
- Measured serum and tissue concentrations of nitrites, nitrates, and S-nitrosylated proteins.
- Utilized NOS inhibitors and peritoneal macrophage assays to evaluate NO's functional role.
Main Results:
- NOS activity and mRNA expression were upregulated in the liver remnant after PH.
- Nitrate and S-nitrosylated protein levels increased post-PH, correlating with NOS activity.
- NO inhibition affected DNA ploidy patterns, and hepatectomy serum induced NOS in macrophages.
Conclusions:
- Nitric oxide (NO) is implicated in the early stages of liver regeneration after partial hepatectomy.
- Macrophages appear to be primed by hepatectomy, contributing to NO-mediated signaling.
- NO may be a key regulator in the dynamic processes of liver repair.