Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hybridoma stability

F J Castillo1, L J Mullen, B C Grant

  • 1Xoma Corporation, Berkeley, CA.

Developments in Biological Standardization
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of skeletal muscle vasodilatory pathway in adaptive thermogenesis in Sunit Mongolian sheep.

Journal of thermal biology·2025
Same author

HIV incidence among non-migrating persons following a household migration event: a population-based, longitudinal study in Uganda.

medRxiv : the preprint server for health sciences·2023
Same author

Activity of imipenem/relebactam against Enterobacterales and Pseudomonas aeruginosa in Spain. SMART 2016-2020.

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2023
Same author

Antimicrobial activity of ceftolozane-tazobactam against Enterobacterales and Pseudomonas aeruginosa recovered during the Study for Monitoring Antimicrobial Resistance Trends (SMART) program in Spain (2016-2018).

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2021
Same author

Evaluation of miLINC to shorten time to treatment for rifampicin-resistant <i>Mycobacterium tuberculosis</i>.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2019
Same author

Monitoring the antimicrobial susceptibility of Gram-negative organisms involved in intraabdominal and urinary tract infections recovered during the SMART study (Spain, 2016 and 2017).

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2019
Same journal

International challenges and public policy issues.

Developments in biological standardization·2000
Same journal

Standardisation and licensing of combination vaccines based on DTP.

Developments in biological standardization·2000
Same journal

The transmissible spongiform encephalopathy agents: concerns and responses of United States regulatory agencies in maintaining the safety of biologics.

Developments in biological standardization·2000
Same journal

Cytokine and growth factor standardization: the need for research to answer modern issues.

Developments in biological standardization·2000
Same journal

Alternatives to the use of animals for bacterial toxins and antitoxins.

Developments in biological standardization·2000
Same journal

Cell substrates: lessons learned and challenges remaining.

Developments in biological standardization·2000
See all related articles

Hybridoma cell lines used for monoclonal antibody (MAb) production demonstrate stability during extended perfusion cultures. This ensures consistent MAb yield and quality, supporting reproducible manufacturing processes.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Biopharmaceutical Manufacturing

Background:

  • Hybridoma cell lines can exhibit instability, including mutations and chromosomal changes, affecting monoclonal antibody (MAb) production.
  • Early-stage hybridomas post-fusion are often unstable, but repeated cloning typically yields stable cell lines.
  • Process variables can impact the yield, quality, and homogeneity of MAb products.

Purpose of the Study:

  • To evaluate the stability of hybridomas and the consistency of MAbs produced during extended high-density perfusion cultures.
  • To assess cell stability by comparing growth and product formation kinetics of bioreactor-recovered cells with fresh cells from working cell banks.
  • To determine MAb product consistency through various biochemical and functional assays.

Main Methods:

Related Experiment Videos

  • Assessed hybridoma cell stability by monitoring growth and product formation kinetics during perfusion culture.
  • Compared bioreactor-recovered cells with Manufacturer's Working Cell Bank cells.
  • Evaluated MAb consistency using SDS-PAGE, IEF, HPLC, peptide mapping, N-terminal sequencing, carbohydrate analysis, and binding assays.

Main Results:

  • Several murine hybridomas maintained stability throughout multi-month perfusion runs.
  • All employed criteria confirmed the stability of the hybridomas and the consistency of the MAbs produced.
  • No significant degradation in cell growth or MAb quality was observed during extended culture.

Conclusions:

  • Extended hollow fiber perfusion cultures are viable for reproducible murine MAb production.
  • Selecting stable hybridoma clones is crucial for controlling stability during manufacturing.
  • Understanding process variable effects on MAb quantity and quality is key to hybridoma stability.