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[Hematopoietic stem cells of the human. Function and morphology]
C Wickenhauser1, J Thiele, T Kümmel
1Institut für Pathologie, Universität zu Köln.
Der Pathologe
|January 1, 1995
Summary
Hematopoietic stem cells expressing CD34 (CD34+) are crucial for blood cell development. Methods to isolate these CD34+ cells are vital for cancer treatments like stem cell transplantation.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Context:
- Pluripotential hematopoietic stem cells (HSCs) and their lineage-restricted progeny, progenitor cells, are identified by CD34 expression.
- CD34+ cells can be enriched and characterized using cell separation techniques like fluorescence staining or ferro-magnetic particles bound to CD34 antibodies.
Purpose:
- To characterize CD34-positive (CD34+) stem and progenitor cells from peripheral blood and bone marrow.
- To compare the cellular and ultrastructural properties of CD34+ cells from different sources.
- To highlight the role of CD34+ progenitor cell enrichment in modern oncology.
Summary:
- CD34+ stem cells from peripheral blood are a uniform population resembling large stimulated lymphocytes, with a lymphoid character and few organelles.
- Bone marrow-derived CD34+ stem cells are more heterogeneous, larger, and exhibit more varied nuclear and cytoplasmic features.
- Both peripheral blood and bone marrow CD34+ progenitors co-express CD43 and CD45, with varying levels of lineage-specific markers like CD33, CD38, and CD20.
Impact:
- Immuno- and ferromagnetic enrichment methods for CD34+ progenitor cells are innovative tools in oncology.
- These techniques are important for autologous and heterologous stem cell transplantation in treating hematological malignancies and advanced tumors.