Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tyrosine phosphorylation in the human duodenum

D Kelleher1, A Murphy, O Sheils

  • 1Department of Clinical Medicine, Trinity College, Dublin, Ireland.

Gut
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic risk-targeted islet autoantibody screening for presymptomatic type 1 diabetes in adults.

The lancet. Diabetes & endocrinology·2025
Same author

Multi-criteria analysis of strategies towards sustainable recycling of phosphorus from sewage sludge in Austria.

Journal of environmental management·2024
Same author

Exploring management and outcomes of elderly patients with glioblastoma using data from two randomised trials (GEINO1401/EX-TEM).

Journal of neuro-oncology·2024
Same author

Trends in Paediatric Emergency Medical Presentations.

Irish medical journal·2024
Same author

Correction to: A combined analysis of two prospective randomised studies exploring the impact of extended post-radiation temozolomide on survival outcomes in newly diagnosed glioblastoma.

Journal of neuro-oncology·2024
Same author

A combined analysis of two prospective randomised studies exploring the impact of extended post-radiation temozolomide on survival outcomes in newly diagnosed glioblastoma.

Journal of neuro-oncology·2023

Tyrosine phosphorylation in the duodenum is mainly in villous cells, not crypt cells. This suggests tyrosine kinase activity doesn

Area of Science:

  • Gastroenterology
  • Cell Biology
  • Immunology

Background:

  • Growth factor receptors, like the epidermal growth factor receptor, utilize tyrosine kinase activity.
  • Understanding tyrosine phosphorylation is crucial for studying cellular processes in the duodenum.

Purpose of the Study:

  • To investigate tyrosine phosphorylation levels in normal and coeliac duodenal tissues.
  • To determine the role of tyrosine kinase activity in duodenal cell proliferation.

Main Methods:

  • Developed a flow cytometric assay using a specific antibody for phosphorylated tyrosine.
  • Analyzed intraepithelial lymphocytes and epithelial cells from duodenal biopsies.
  • Utilized immunohistochemistry on coeliac and control duodenal specimens.

Related Experiment Videos

Main Results:

  • Tyrosine phosphorylation was detected in duodenal enterocytes, enhanced by epidermal growth factor.
  • Phosphorylation decreased from villus to crypt, being undetectable in crypt enterocytes.
  • Coeliac duodenum showed absent crypt phosphorylation but increased phosphorylation in infiltrating T cells.

Conclusions:

  • Duodenal tyrosine phosphorylation is localized to the non-proliferative villous epithelium.
  • Tyrosine kinase activity appears not to regulate human duodenal crypt cell proliferation in normal or coeliac states.