Pb2+ activates potassium currents in bovine adrenal chromaffin cells

Inorganic lead (Pb2+) is a potent environmental toxin which adversely affects several aspects of neuronal and secretory cell function. In this report, we provide evidence that at subnanomolar concentrations, Pb2+ activates the outward K+ currents in bovine adrenal chromaffin cells. Whole-cell patch clamp combined with intracellular perfusion was employed to monitor outward K+ currents in bovine chromaffin cells before and after intracellular application of EGTA-Pb buffers. Intracellular Pb2+ > or = 10(-10) M enhanced the K(+)-currents in a concentration dependent manner, with apparent K0.5 approximately equal to 5 x 10(-10) M. Extracellular application of 40 nM Charybdotoxin (ChTX) blocked the Pb(2+)-dependent component of outward currents, suggesting that Pb2+ activates the large conductance Ca(2+)-dependent K+ channels.