Related Experiment Video

Updated: Oct 9, 2026

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

An adenovirus type 5 early region 1B-encoded CTL epitope-mediating tumor eradication by CTL clones is down-modulated

R E Toes1, R Offringa, R J Blom

  • 1Department of Immunohematology and Blood Bank, University Hospital Leiden, The Netherlands.

Journal of Immunology (Baltimore, Md. : 1950)
|April 1, 1995
PubMed

Insights

Adenovirus type 5 transformed mouse cells elicit a potent cytotoxic T lymphocyte (CTL) response. Researchers identified a new Ad5E1B epitope, demonstrating that targeting multiple tumor epitopes can enhance cancer immunotherapy.

Area of Science:

  • Immunology
  • Oncology
  • Virology

Background:

  • Adenovirus type 5 (Ad5) transformed mouse embryo cells (MECs) are highly immunogenic.
  • Cytotoxic T lymphocytes (CTLs) cloned from Ad5E1 MEC-immunized mice eradicated tumors.
  • These CTLs targeted an Ad5E1A-encoded epitope presented by H-2Db.

Purpose of the Study:

  • To investigate CTL responses against Ad5E1 MECs with mutated Ad5E1A epitopes.
  • To identify novel CTL epitopes on Ad5E1-transformed tumor cells.
  • To explore the potential of targeting multiple tumor epitopes for cancer immunotherapy.

Main Methods:

  • Generation of Ad5E1 MECs with mutated Ad5E1A-encoded epitopes.
  • Induction of CTL responses in immunocompetent mice.
  • Cloning and characterization of CTLs against mutant Ad5E1 MECs.
  • Assessment of tumor eradication in nude mice.

Main Results:

  • Mutant Ad5E1 MECs induced a strong CTL response.
  • CTL clones recognized an Ad5E1B-encoded epitope (VNIRNCCYI) presented by H-2Db.
  • Ad5E1-transformed tumor cells express at least two distinct CTL epitopes.
  • Both Ad5E1A- and Ad5E1B-specific CTLs eradicated established Ad5E1-induced tumors in vivo.
  • Activated ras oncogene diminished lysis by Ad5E1B-specific CTLs.

Conclusions:

  • Ad5E1-transformed tumor cells express multiple CTL epitopes, including Ad5E1A and Ad5E1B.
  • Targeting distinct CTL epitopes on tumors holds promise for effective cancer immunotherapy.
  • The ras oncogene may influence the immunogenicity of Ad5E1B-specific CTL targets.

More Related Videos

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer07:13

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Published on: March 26, 2014

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches13:36

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...