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Stabilization of neurofilament transcripts during postnatal development

M L Schwartz1, P S Shneidman, J Bruce

  • 1Division of Neuropathology, University of Pennsylvania Medical School, Philadelphia 19104-6079.

Brain Research. Molecular Brain Research
|December 1, 1994
PubMed
Summary
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Neurofilament (NF) mRNA stability increases with postnatal development in sensory neurons. This stabilization contributes to coordinated upregulation of NF-L, NF-M, and NF-H subunits during development.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Neurofilament (NF) mRNAs are long-lived transcripts in primary sensory neurons.
  • These transcripts undergo transcription-dependent destabilization in primary culture.
  • Stabilization of NF transcripts may coordinate and increase NF expression levels.

Purpose of the Study:

  • To examine the stabilities of the three NF subunit mRNAs (NF-L, NF-M, NF-H) in postnatal dorsal root ganglia (DRG) cultures.
  • To determine if increased NF mRNA stability explains the coordinate postnatal upregulation of NF subunits.
  • To investigate the developmental acquisition of NF transcript stabilization mechanisms.

Main Methods:

  • Primary cultures of rat dorsal root ganglia (DRG) were established from postnatal day 2 (P2), P16, and P30 rats.

Related Experiment Videos

  • The stability of NF-L, NF-M, and NF-H mRNAs was assessed over time (8 and 16 hours).
  • Experiments were conducted in the presence and absence of actinomycin to evaluate transcription-dependent destabilization.

    • Main Results:

    • NF mRNAs were rapidly lost in P2 rat DRG cultures (8-16 hours).
    • NF mRNA loss was significantly reduced in cultures from P16 and P30 rats.
    • Losses of all three NF mRNAs occurred simultaneously in P2 cultures, irrespective of actinomycin presence.
    • These findings indicate developmental changes in NF mRNA stability.

    Conclusions:

    • Stabilization of NF transcripts contributes to high and coordinate NF expression levels.
    • Components of the NF transcript stabilization process are acquired during postnatal development.
    • This developmental stabilization mechanism is crucial for regulating neurofilament expression in sensory neurons.