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Mechanisms of regulatory volume decrease in nonpigmented human ciliary epithelial cells

J S Adorante¹, P M Cala

¹Allergan, Inc., Irvine 92715.

The American Journal of Physiology

|March 1, 1995

Summary

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Human NPE cells regulate volume via K+ and Cl- channels. Swelling triggers calcium increases, modulating ion efflux for volume recovery, crucial for ciliary epithelium function.

Area of Science:

Cell Biology
Physiology
Ocular Science

Background:

Cell volume regulation is critical for epithelial function.
Non-pigmented ciliary epithelium (NPE) cells play a role in aqueous humor production.
Ion and water flux are linked to cell volume control.

Purpose of the Study:

Investigate ion efflux pathways in NPE cells.
Characterize the mechanisms of regulatory volume decrease (RVD).
Determine the role of intracellular calcium in RVD.

Main Methods:

Utilized cultured human NPE cell line.
Induced osmotic swelling and monitored volume recovery.
Assessed ion channel activity using specific blockers and ionophores.
Measured intracellular calcium concentration ([Ca2+]i) changes.

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Main Results:

NPE cell swelling triggered regulatory volume decrease (RVD).
RVD was dependent on K+ and Cl- conductances, inhibited by specific channel blockers.
Osmotic swelling increased intracellular calcium concentration ([Ca2+]i).
Calcium signaling and influx were essential for RVD.

Conclusions:

RVD in NPE cells is mediated by distinct K+ and Cl- channels.
Swelling-induced intracellular calcium increases modulate net ion efflux.
These findings elucidate solute and water transport pathways in the ciliary epithelium.