Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peptide ligands for integrin alpha v beta 3 selected from random phage display libraries

J M Healy1, O Murayama, T Maeda

  • 1Protein Engineering Research Institute, Osaka, Japan.

Biochemistry
|March 28, 1995
PubMed
Summary

Integrin alpha v beta 3 recognizes RGD-containing proteins. Phage display identified diverse RGD peptides and a novel vitronectin-derived sequence, revealing the receptor

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New measurement system for the spin correlation coefficients in deuteron-proton elastic scattering.

The European physical journal. A, Hadrons and nuclei·2026
Same author

Precise Spectroscopy of the 3n and 3p Systems via the ^{3}H(t, ^{3}He)3n and ^{3}He(^{3}He, t)3p Reactions at Intermediate Energies.

Physical review letters·2024
Same author

Electron Heating in High Mach Number Collisionless Shocks.

Physical review letters·2024
Same author

Effect of tibial component alignment on knee kinematics and ligament tension in medial unicompartmental knee arthroplasty.

Bone & joint research·2019
Same author

Fruit and Vegetable Consumption and Frailty: A Systematic Review.

The journal of nutrition, health & aging·2018
Same author

Full 24-month treatment course with daily teriparatide: a mechanistic insight.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2016

Area of Science:

  • Molecular biology
  • Cell adhesion
  • Protein-protein interactions

Background:

  • Integrin alpha v beta 3 is a key receptor mediating cell adhesion to extracellular matrix proteins.
  • It recognizes the arginine-glycine-aspartic acid (RGD) motif present in various adhesive proteins like vitronectin and fibronectin.

Purpose of the Study:

  • To investigate the molecular recognition mechanisms of integrin alpha v beta 3.
  • To identify novel peptide ligands that bind to this integrin.

Main Methods:

  • Utilized phage display technology to select ligands from large random peptide libraries.
  • Analyzed the binding specificity and sequence composition of selected peptides.

Main Results:

  • Identified RGD-containing peptides as primary ligands for integrin alpha v beta 3.

Related Experiment Videos

  • Observed significant heterogeneity in amino acids flanking the RGD motif, indicating receptor tolerance.
  • Discovered a novel binding sequence, a vitronectin-derived tetrapeptide, suggesting a potential synergistic binding site.

    • Conclusions:

    • Integrin alpha v beta 3 exhibits broad specificity for RGD-containing peptides.
    • The receptor's versatility supports its role in binding diverse extracellular matrix proteins.
    • A novel synergistic binding site in vitronectin may cooperate with the RGD motif for enhanced molecular recognition.